We read with interest the article by Du, et al., on metabolomic changes and homeostatic regulation in three TYMP-deficient patients assessed by targeted and non-targeted metabolomic techniques applied to serum and skin fibroblasts [Du, J. et al., 2023]. The results were compared to those of three m.3243A>G carriers and four healthy controls [Du, J. et al., 2023]. Specific changes in nucleosides, bile acids, and steroid metabolism have been found in the plasma of MNGIE patients [Du, J. et al., 2023]. In skin fibroblasts mitochondrial dysfunction, decreased cholesterol metabolism and decreased fatty acid biosynthesis and degradation have been found [Du, J. et al., 2023]. The study is excellent but has limitations that should be discussed.

The first limitation of the study is that secondary changes in mtDNA caused by TYMP deficiency have not been examined [Du, J. et al., 2023]. Since TYMP variants can cause multiple mtDNA deletions, mtDNA point mutations, and even mtDNA depletion due to defective mtDNA replication and since the secondary mtDNA defects vary between the types of TYMP variants but also between patients carrying the same TYMP variant, each individual MNGIE patient represents a unique phenotype that also concerns the metabolic profile.

A second limitation is that the current medications that the included patients were regularly taking were not provided. Since all included patients were multimorbid, it is very likely that they all received regular medication. Since medications can strongly influence the blood composition of metabolites examined in the index study, it is imperative to be aware of them and to include them in the discussion of the results.

A third limitation is that dependence of serum metabolite composition on the underlying mutation was also not considered in the discussion. Differences  between  MNGIE  patients1/2  and patient-3 could simply be due to the different genetic defects. Differences between MNGIE patients and MELAS patients can be explained simply by the different mutated genes.